Functional and molecular features of the calmodulin-interacting protein IQCG required for haematopoiesis in zebrafish

Li-Ting Chen, Wen-Xue Liang, Shuo Chen, Ren-Ke Li, Jue-Ling Tan, Peng-Fei Xu, Liu-Fei Luo, Lei Wang, Shan-He Yu, Guoyu Meng, Keqin Kathy Li, Ting-Xi Liu, Zhu Chen () and Sai-Juan Chen ()
Additional contact information
Li-Ting Chen: State Key Laboratory of Medical Genomics and Shanghai, Institute of Hematology, Rui Jin Hospital affiliated to Shanghai Jiao Tong University (SITU) School of Medicine
Wen-Xue Liang: Central Laboratory, Lianyungang First People’s Hospital
Shuo Chen: Ludwig Institute for Cancer Research, Old Road Campus Research Building, University of Oxford
Ren-Ke Li: State Key Laboratory of Medical Genomics and Shanghai, Institute of Hematology, Rui Jin Hospital affiliated to Shanghai Jiao Tong University (SITU) School of Medicine
Jue-Ling Tan: State Key Laboratory of Medical Genomics and Shanghai, Institute of Hematology, Rui Jin Hospital affiliated to Shanghai Jiao Tong University (SITU) School of Medicine
Peng-Fei Xu: State Key Laboratory of Medical Genomics and Shanghai, Institute of Hematology, Rui Jin Hospital affiliated to Shanghai Jiao Tong University (SITU) School of Medicine
Liu-Fei Luo: State Key Laboratory of Medical Genomics and Shanghai, Institute of Hematology, Rui Jin Hospital affiliated to Shanghai Jiao Tong University (SITU) School of Medicine
Lei Wang: Institute of Health Sciences, Shanghai Institutes for Biological Sciences and Graduate School, Chinese Academy of Sciences and SITU School of Medicine
Shan-He Yu: State Key Laboratory of Medical Genomics and Shanghai, Institute of Hematology, Rui Jin Hospital affiliated to Shanghai Jiao Tong University (SITU) School of Medicine
Guoyu Meng: State Key Laboratory of Medical Genomics and Shanghai, Institute of Hematology, Rui Jin Hospital affiliated to Shanghai Jiao Tong University (SITU) School of Medicine
Keqin Kathy Li: State Key Laboratory of Medical Genomics and Shanghai, Institute of Hematology, Rui Jin Hospital affiliated to Shanghai Jiao Tong University (SITU) School of Medicine
Ting-Xi Liu: State Key Laboratory of Medical Genomics and Shanghai, Institute of Hematology, Rui Jin Hospital affiliated to Shanghai Jiao Tong University (SITU) School of Medicine
Zhu Chen: State Key Laboratory of Medical Genomics and Shanghai, Institute of Hematology, Rui Jin Hospital affiliated to Shanghai Jiao Tong University (SITU) School of Medicine
Sai-Juan Chen: State Key Laboratory of Medical Genomics and Shanghai, Institute of Hematology, Rui Jin Hospital affiliated to Shanghai Jiao Tong University (SITU) School of Medicine

Nature Communications, 2014, vol. 5, issue 1, 1-12

Abstract: Abstract We previously reported a fusion protein NUP98–IQCG in an acute leukaemia, which functions as an aberrant regulator of transcriptional expression, yet the structure and function of IQCG have not been characterized. Here we use zebrafish to investigate the role of iqcg in haematopoietic development, and find that the numbers of haematopoietic stem cells and multilineage-differentiated cells are reduced in iqcg-deficient embryos. Mechanistically, IQCG binds to calmodulin (CaM) and acts as a molecule upstream of CaM-dependent kinase IV (CaMKIV). Crystal structures of complexes between CaM and IQ domain of IQCG reveal dual CaM-binding footprints in this motif, and provide a structural basis for a higher CaM–IQCG affinity when deprived of calcium. The results collectively allow us to understand IQCG-mediated calcium signalling in haematopoiesis, and propose a model in which IQCG stores CaM at low cytoplasmic calcium concentrations, and releases CaM to activate CaMKIV when calcium level rises.

Date: 2014
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/ncomms4811 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4811

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms4811

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().