Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology

Fayza Daboussi (), Sophie Leduc, Alan Maréchal, Gwendoline Dubois, Valérie Guyot, Christophe Perez-Michaut, Alberto Amato, Angela Falciatore, Alexandre Juillerat, Marine Beurdeley, Daniel F. Voytas, Laurent Cavarec and Philippe Duchateau
Additional contact information
Fayza Daboussi: Cellectis S.A., 8 rue de la Croix de Jarry
Sophie Leduc: Cellectis S.A., 8 rue de la Croix de Jarry
Alan Maréchal: Cellectis S.A., 8 rue de la Croix de Jarry
Gwendoline Dubois: Cellectis S.A., 8 rue de la Croix de Jarry
Valérie Guyot: Cellectis S.A., 8 rue de la Croix de Jarry
Christophe Perez-Michaut: Cellectis S.A., 8 rue de la Croix de Jarry
Alberto Amato: Cellectis S.A., 8 rue de la Croix de Jarry
Angela Falciatore: Université Pierre et Marie Curie, UMR7238, CNRS-UPMC
Alexandre Juillerat: Cellectis S.A., 8 rue de la Croix de Jarry
Marine Beurdeley: Cellectis S.A., 8 rue de la Croix de Jarry
Daniel F. Voytas: Cellectis plant sciences
Laurent Cavarec: Cellectis S.A., 8 rue de la Croix de Jarry
Philippe Duchateau: Cellectis S.A., 8 rue de la Croix de Jarry

Nature Communications, 2014, vol. 5, issue 1, 1-7

Abstract: Abstract Diatoms, a major group of photosynthetic microalgae, have a high biotechnological potential that has not been fully exploited because of the paucity of available genetic tools. Here we demonstrate targeted and stable modifications of the genome of the marine diatom Phaeodactylum tricornutum, using both meganucleases and TALE nucleases. When nuclease-encoding constructs are co-transformed with a selectable marker, high frequencies of genome modifications are readily attained with 56 and 27% of the colonies exhibiting targeted mutagenesis or targeted gene insertion, respectively. The generation of an enhanced lipid-producing strain (45-fold increase in triacylglycerol accumulation) through the disruption of the UDP-glucose pyrophosphorylase gene exemplifies the power of genome engineering to harness diatoms for biofuel production.

Date: 2014
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DOI: 10.1038/ncomms4831

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