Overproduction of stomatal lineage cells in Arabidopsis mutants defective in active DNA demethylation

Chizuko Yamamuro, Daisuke Miki, Zhimin Zheng, Jun Ma, Jing Wang, Zhenbiao Yang, Juan Dong and Jian-Kang Zhu ()
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Chizuko Yamamuro: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Daisuke Miki: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Zhimin Zheng: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Jun Ma: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Jing Wang: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Zhenbiao Yang: Center for Plant Cell Biology, University of California
Juan Dong: Waksman Institute of Microbiology, Rutgers University
Jian-Kang Zhu: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

Nature Communications, 2014, vol. 5, issue 1, 1-7

Abstract: Abstract DNA methylation is a reversible epigenetic mark regulating genome stability and function in many eukaryotes. In Arabidopsis, active DNA demethylation depends on the function of the ROS1 subfamily of genes that encode 5-methylcytosine DNA glycosylases/lyases. ROS1-mediated DNA demethylation plays a critical role in the regulation of transgenes, transposable elements and some endogenous genes; however, there have been no reports of clear developmental phenotypes in ros1 mutant plants. Here we report that, in the ros1 mutant, the promoter region of the peptide ligand gene EPF2 is hypermethylated, which greatly reduces EPF2 expression and thereby leads to a phenotype of overproduction of stomatal lineage cells. EPF2 gene expression in ros1 is restored and the defective epidermal cell patterning is suppressed by mutations in genes in the RNA-directed DNA methylation pathway. Our results show that active DNA demethylation combats the activity of RNA-directed DNA methylation to influence the initiation of stomatal lineage cells.

Date: 2014
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DOI: 10.1038/ncomms5062

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