ATG5 defines a phagophore domain connected to the endoplasmic reticulum during autophagosome formation in plants

Romain Le Bars, Jessica Marion, Rémi Le Borgne, Béatrice Satiat-Jeunemaitre () and Michele Wolfe Bianchi ()
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Romain Le Bars: Laboratoire Dynamique de la Compartimentation Cellulaire, CNRS UPR2355, Institut des Sciences du Végétal, Centre de Recherche de Gif (FRC3115), Saclay Plant Sciences
Jessica Marion: Laboratoire Dynamique de la Compartimentation Cellulaire, CNRS UPR2355, Institut des Sciences du Végétal, Centre de Recherche de Gif (FRC3115), Saclay Plant Sciences
Rémi Le Borgne: Laboratoire Dynamique de la Compartimentation Cellulaire, CNRS UPR2355, Institut des Sciences du Végétal, Centre de Recherche de Gif (FRC3115), Saclay Plant Sciences
Béatrice Satiat-Jeunemaitre: Laboratoire Dynamique de la Compartimentation Cellulaire, CNRS UPR2355, Institut des Sciences du Végétal, Centre de Recherche de Gif (FRC3115), Saclay Plant Sciences
Michele Wolfe Bianchi: Laboratoire Dynamique de la Compartimentation Cellulaire, CNRS UPR2355, Institut des Sciences du Végétal, Centre de Recherche de Gif (FRC3115), Saclay Plant Sciences

Nature Communications, 2014, vol. 5, issue 1, 1-10

Abstract: Abstract Autophagosomes are the organelles responsible for macroautophagy and arise, in yeast and animals, from the sealing of a cup-shaped double-membrane precursor, the phagophore. How the phagophore is generated and grows into a sealed autophagosome is still not clear in detail, and unknown in plants. This is due, in part, to the scarcity of structurally informative, real-time imaging data of the required protein machinery at the phagophore formation site. Here we find that in intact living Arabidopsis tissue, autophagy-related protein ATG5, which is essential for autophagosome formation, is present at the phagophore site from early, sub-resolution stages and later defines a torus-shaped structure on a flat cisternal early phagophore. Movement and expansion of this structure are accompanied by the underlying endoplasmic reticulum, suggesting tight connections between the two compartments. Detailed real-time and 3D imaging of the growing phagophore are leveraged to propose a model for autophagosome formation in plants.

Date: 2014
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DOI: 10.1038/ncomms5121

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