Structural insight into SUMO chain recognition and manipulation by the ubiquitin ligase RNF4

Yingqi Xu, Anna Plechanovová, Peter Simpson, Jan Marchant, Orsolya Leidecker, Sebastian Kraatz, Ronald T. Hay () and Steve J. Matthews ()
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Yingqi Xu: Centre for Structural Biology, Imperial College London
Anna Plechanovová: Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee
Peter Simpson: Centre for Structural Biology, Imperial College London
Jan Marchant: Centre for Structural Biology, Imperial College London
Orsolya Leidecker: Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee
Sebastian Kraatz: Centre for Structural Biology, Imperial College London
Ronald T. Hay: Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee
Steve J. Matthews: Centre for Structural Biology, Imperial College London

Nature Communications, 2014, vol. 5, issue 1, 1-12

Abstract: Abstract The small ubiquitin-like modifier (SUMO) can form polymeric chains that are important signals in cellular processes such as meiosis, genome maintenance and stress response. The SUMO-targeted ubiquitin ligase RNF4 engages with SUMO chains on linked substrates and catalyses their ubiquitination, which targets substrates for proteasomal degradation. Here we use a segmental labelling approach combined with solution nuclear magnetic resonance (NMR) spectroscopy and biochemical characterization to reveal how RNF4 manipulates the conformation of the SUMO chain, thereby facilitating optimal delivery of the distal SUMO domain for ubiquitin transfer.

Date: 2014
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5217

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DOI: 10.1038/ncomms5217

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