Offspring production with sperm grown in vitro from cryopreserved testis tissues

Tetsuhiro Yokonishi, Takuya Sato, Mitsuru Komeya, Kumiko Katagiri, Yoshinobu Kubota, Kazuhiko Nakabayashi, Kenichiro Hata, Kimiko Inoue, Narumi Ogonuki, Atsuo Ogura and Takehiko Ogawa ()
Additional contact information
Tetsuhiro Yokonishi: Yokohama City University Graduate School of Medicine
Takuya Sato: Laboratory of Proteomics, Institute of Molecular Medicine and Life Science, Yokohama City University Association of Medical Science
Mitsuru Komeya: Yokohama City University Graduate School of Medicine
Kumiko Katagiri: Laboratory of Proteomics, Institute of Molecular Medicine and Life Science, Yokohama City University Association of Medical Science
Yoshinobu Kubota: Yokohama City University Graduate School of Medicine
Kazuhiko Nakabayashi: National Research Institute for Child Health and Development
Kenichiro Hata: National Research Institute for Child Health and Development
Kimiko Inoue: RIKEN, Bioresource Center
Narumi Ogonuki: RIKEN, Bioresource Center
Atsuo Ogura: RIKEN, Bioresource Center
Takehiko Ogawa: Yokohama City University Graduate School of Medicine

Nature Communications, 2014, vol. 5, issue 1, 1-6

Abstract: Abstract With the increasing cure rate of paediatric cancers, infertility, as one of the adverse effects of treatments, has become an important concern for patients and their families. Since semen cryopreservation is applicable only for post-pubertal patients, alternative pre-pubertal measures are necessary. Here we demonstrate that testis tissue cryopreservation is a realistic measure for preserving the fertility of an individual. Testis tissues of neonatal mice were cryopreserved either by slow freezing or by vitrification. After thawing, they were cultured on agarose gel and showed spermatogenesis up to sperm formation. Microinsemination was performed with round spermatids and sperm, leading to eight offspring in total. They grew healthily and produced progeny upon natural mating between them. This strategy, the cryopreservation of testis tissues followed by in vitro spermatogenesis, is promising to preserve the fertility of male paediatric cancer patients in the future.

Date: 2014
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms5320 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5320

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms5320

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().