Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis

Hanchuan Peng (), Jianyong Tang, Hang Xiao, Alessandro Bria, Jianlong Zhou, Victoria Butler, Zhi Zhou, Paloma T. Gonzalez-Bellido, Seung W. Oh, Jichao Chen, Ananya Mitra, Richard W. Tsien, Hongkui Zeng, Giorgio A. Ascoli, Giulio Iannello, Michael Hawrylycz, Eugene Myers and Fuhui Long
Additional contact information
Hanchuan Peng: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA
Jianyong Tang: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA
Hang Xiao: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA
Alessandro Bria: Integrated Research Centre, University Campus Bio-Medico of Rome
Jianlong Zhou: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA
Victoria Butler: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA
Zhi Zhou: Allen Institute for Brain Science, 551 North 34th Street, Suite 200, Seattle, Washington 98103, USA
Paloma T. Gonzalez-Bellido: Development and Neuroscience, University of Cambridge
Seung W. Oh: Allen Institute for Brain Science, 551 North 34th Street, Suite 200, Seattle, Washington 98103, USA
Jichao Chen: M. D. Anderson Cancer Center
Ananya Mitra: Stanford University School of Medicine
Richard W. Tsien: Stanford University School of Medicine
Hongkui Zeng: Allen Institute for Brain Science, 551 North 34th Street, Suite 200, Seattle, Washington 98103, USA
Giorgio A. Ascoli: Krasnow Institute for Advanced Study, George Mason University
Giulio Iannello: Integrated Research Centre, University Campus Bio-Medico of Rome
Michael Hawrylycz: Allen Institute for Brain Science, 551 North 34th Street, Suite 200, Seattle, Washington 98103, USA
Eugene Myers: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA
Fuhui Long: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA

Nature Communications, 2014, vol. 5, issue 1, 1-13

Abstract: Abstract Three-dimensional (3D) bioimaging, visualization and data analysis are in strong need of powerful 3D exploration techniques. We develop virtual finger (VF) to generate 3D curves, points and regions-of-interest in the 3D space of a volumetric image with a single finger operation, such as a computer mouse stroke, or click or zoom from the 2D-projection plane of an image as visualized with a computer. VF provides efficient methods for acquisition, visualization and analysis of 3D images for roundworm, fruitfly, dragonfly, mouse, rat and human. Specifically, VF enables instant 3D optical zoom-in imaging, 3D free-form optical microsurgery, and 3D visualization and annotation of terabytes of whole-brain image volumes. VF also leads to orders of magnitude better efficiency of automated 3D reconstruction of neurons and similar biostructures over our previous systems. We use VF to generate from images of 1,107 Drosophila GAL4 lines a projectome of a Drosophila brain.

Date: 2014
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DOI: 10.1038/ncomms5342

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