An infectious bat-derived chimeric influenza virus harbouring the entry machinery of an influenza A virus

Mindaugas Juozapaitis, Étori Aguiar Moreira, Ignacio Mena, Sebastian Giese, David Riegger, Anne Pohlmann, Dirk Höper, Gert Zimmer, Martin Beer, Adolfo García-Sastre and Martin Schwemmle ()
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Mindaugas Juozapaitis: Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany
Étori Aguiar Moreira: Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany
Ignacio Mena: Icahn School of Medicine at Mount Sinai
Sebastian Giese: Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany
David Riegger: Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany
Anne Pohlmann: Institute of Diagnostic Virology, Friedrich-Loeffler-Institute
Dirk Höper: Institute of Diagnostic Virology, Friedrich-Loeffler-Institute
Gert Zimmer: Institute of Virology and Immunology (IVI)
Martin Beer: Institute of Diagnostic Virology, Friedrich-Loeffler-Institute
Adolfo García-Sastre: Icahn School of Medicine at Mount Sinai
Martin Schwemmle: Institute of Virology, Center for Microbiology and Hygiene, University Medical Center Freiburg, Hermann-Herder-Street 11, 79104 Freiburg, Germany

Nature Communications, 2014, vol. 5, issue 1, 1-8

Abstract: Abstract In 2012, the complete genomic sequence of a new and potentially harmful influenza A-like virus from bats (H17N10) was identified. However, infectious influenza virus was neither isolated from infected bats nor reconstituted, impeding further characterization of this virus. Here we show the generation of an infectious chimeric virus containing six out of the eight bat virus genes, with the remaining two genes encoding the haemagglutinin and neuraminidase proteins of a prototypic influenza A virus. This engineered virus replicates well in a broad range of mammalian cell cultures, human primary airway epithelial cells and mice, but poorly in avian cells and chicken embryos without further adaptation. Importantly, the bat chimeric virus is unable to reassort with other influenza A viruses. Although our data do not exclude the possibility of zoonotic transmission of bat influenza viruses into the human population, they indicate that multiple barriers exist that makes this an unlikely event.

Date: 2014
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DOI: 10.1038/ncomms5448

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