Reconstituting ring-rafts in bud-mimicking topography of model membranes

Yong-Sang Ryu, In-Ho Lee, Jeng-Hun Suh, Seung Chul Park, Soojung Oh, Luke R. Jordan, Nathan J. Wittenberg, Sang-Hyun Oh, Noo Li Jeon, Byoungho Lee, Atul N. Parikh () and Sin-Doo Lee ()
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Yong-Sang Ryu: School of Electrical Engineering #032, Seoul National University
In-Ho Lee: School of Electrical Engineering #032, Seoul National University
Jeng-Hun Suh: School of Electrical Engineering #032, Seoul National University
Seung Chul Park: School of Electrical Engineering #032, Seoul National University
Soojung Oh: World Class University (WCU) Program of Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University
Luke R. Jordan: University of Minnesota
Nathan J. Wittenberg: University of Minnesota
Sang-Hyun Oh: University of Minnesota
Noo Li Jeon: World Class University (WCU) Program of Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University
Byoungho Lee: School of Electrical Engineering #032, Seoul National University
Atul N. Parikh: University of California
Sin-Doo Lee: School of Electrical Engineering #032, Seoul National University

Nature Communications, 2014, vol. 5, issue 1, 1-8

Abstract: Abstract During vesicular trafficking and release of enveloped viruses, the budding and fission processes dynamically remodel the donor cell membrane in a protein- or a lipid-mediated manner. In all cases, in addition to the generation or relief of the curvature stress, the buds recruit specific lipids and proteins from the donor membrane through restricted diffusion for the development of a ring-type raft domain of closed topology. Here, by reconstituting the bud topography in a model membrane, we demonstrate the preferential localization of cholesterol- and sphingomyelin-enriched microdomains in the collar band of the bud-neck interfaced with the donor membrane. The geometrical approach to the recapitulation of the dynamic membrane reorganization, resulting from the local radii of curvatures from nanometre-to-micrometre scales, offers important clues for understanding the active roles of the bud topography in the sorting and migration machinery of key signalling proteins involved in membrane budding.

Date: 2014
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DOI: 10.1038/ncomms5507

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