O-GlcNAc-mediated interaction between VER2 and TaGRP2 elicits TaVRN1 mRNA accumulation during vernalization in winter wheat

Jun Xiao, Shujuan Xu, Chunhua Li, Yunyuan Xu, Lijing Xing, Yuda Niu, Qing Huan, Yimiao Tang, Changping Zhao, Doris Wagner, Caixia Gao and Kang Chong ()
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Jun Xiao: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences
Shujuan Xu: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences
Chunhua Li: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences
Yunyuan Xu: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences
Lijing Xing: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences
Yuda Niu: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences
Qing Huan: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences
Yimiao Tang: Hybrid Wheat Research Center, Beijing Academy of Agriculture and Forestry Sciences
Changping Zhao: Hybrid Wheat Research Center, Beijing Academy of Agriculture and Forestry Sciences
Doris Wagner: University of Pennsylvania
Caixia Gao: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
Kang Chong: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences

Nature Communications, 2014, vol. 5, issue 1, 1-13

Abstract: Abstract Vernalization, sensing of prolonged cold, is important for seasonal flowering in eudicots and monocots. While vernalization silences a repressor (FLC, MADS-box transcription factor) in eudicots, it induces an activator (TaVRN1, an AP1 clade MADS-box transcription factor) in monocots. The mechanism for TaVRN1 induction during vernalization is not well understood. Here we reveal a novel mechanism for controlling TaVRN1 mRNA accumulation in response to prolonged cold sensing in wheat. The carbohydrate-binding protein VER2, a jacalin lectin, promotes TaVRN1 upregulation by physically interacting with the RNA-binding protein TaGRP2. TaGRP2 binds to TaVRN1 pre-mRNA and inhibits TaVRN1 mRNA accumulation. The physical interaction between VER2 and TaGRP2 is controlled by TaGRP2 O-GlcNAc modification, which gradually increases during vernalization. The interaction between VER2 and O-GlcNAc-TaGRP2 reduces TaGRP2 protein accumulation in the nucleus and/or promotes TaGRP2 dissociation from TaVRN1, leading to TaVRN1 mRNA accumulation. Our data reveal a new mechanism for sensing prolonged cold in temperate cereals.

Date: 2014
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DOI: 10.1038/ncomms5572

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