Identification of genetic variants associated with alternative splicing using sQTLseekeR

Jean Monlong, Miquel Calvo, Pedro G. Ferreira and Roderic Guigó ()
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Jean Monlong: Center for Genomic Regulation, Universitat Pompeu Fabra
Miquel Calvo: Facultat de Biologia, Universitat de Barcelona
Pedro G. Ferreira: Center for Genomic Regulation, Universitat Pompeu Fabra
Roderic Guigó: Center for Genomic Regulation, Universitat Pompeu Fabra

Nature Communications, 2014, vol. 5, issue 1, 1-12

Abstract: Abstract Identification of genetic variants affecting splicing in RNA sequencing population studies is still in its infancy. Splicing phenotype is more complex than gene expression and ought to be treated as a multivariate phenotype to be recapitulated completely. Here we represent the splicing pattern of a gene as the distribution of the relative abundances of a gene’s alternative transcript isoforms. We develop a statistical framework that uses a distance-based approach to compute the variability of splicing ratios across observations, and a non-parametric analogue to multivariate analysis of variance. We implement this approach in the R package sQTLseekeR and use it to analyze RNA-Seq data from the Geuvadis project in 465 individuals. We identify hundreds of single nucleotide polymorphisms (SNPs) as splicing QTLs (sQTLs), including some falling in genome-wide association study SNPs. By developing the appropriate metrics, we show that sQTLseekeR compares favorably with existing methods that rely on univariate approaches, predicting variants that behave as expected from mutations affecting splicing.

Date: 2014
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DOI: 10.1038/ncomms5698

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