Ribosomal DNA copy number is coupled with gene expression variation and mitochondrial abundance in humans

John G. Gibbons, Alan T. Branco, Shoukai Yu and Bernardo Lemos ()
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John G. Gibbons: Program in Molecular and Integrative Physiological Sciences, Harvard School of Public Health
Alan T. Branco: Program in Molecular and Integrative Physiological Sciences, Harvard School of Public Health
Shoukai Yu: Program in Molecular and Integrative Physiological Sciences, Harvard School of Public Health
Bernardo Lemos: Program in Molecular and Integrative Physiological Sciences, Harvard School of Public Health

Nature Communications, 2014, vol. 5, issue 1, 1-12

Abstract: Abstract Ribosomes are essential intracellular machines composed of proteins and RNA molecules. The DNA sequences (rDNA) encoding ribosomal RNAs (rRNAs) are tandemly repeated and give origin to the nucleolus. Here we develop a computational method for estimating rDNA dosage (copy number) and mitochondrial DNA abundance using whole-genome short-read DNA sequencing. We estimate these attributes across hundreds of human genomes and their association with global gene expression. The analyses uncover abundant variation in rDNA dosage that is coupled with the expression of hundreds of functionally coherent gene sets. These include associations with genes coding for chromatin components that target the nucleolus, including CTCF and HP1β. Finally, the data show an inverse association between rDNA dosage and mitochondrial DNA abundance that is manifested across genotypes. Our findings uncover a novel and cryptic source of hypervariable genomic diversity with global regulatory consequences (ribosomal eQTL) in humans. The variation provides a mechanism for cellular homeostasis and for rapid and reversible adaptation.

Date: 2014
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DOI: 10.1038/ncomms5850

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