Visualizing the replication of respiratory syncytial virus in cells and in living mice

Marie-Anne Rameix-Welti, Ronan Le Goffic, Pierre-Louis Hervé, Julien Sourimant, Aude Rémot, Sabine Riffault, Qin Yu, Marie Galloux, Elyanne Gault () and Jean-François Eléouët ()
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Marie-Anne Rameix-Welti: Unité de Virologie et Immunologie Moleculaires (UR892), INRA
Ronan Le Goffic: Unité de Virologie et Immunologie Moleculaires (UR892), INRA
Pierre-Louis Hervé: Unité de Virologie et Immunologie Moleculaires (UR892), INRA
Julien Sourimant: Unité de Virologie et Immunologie Moleculaires (UR892), INRA
Aude Rémot: Unité de Virologie et Immunologie Moleculaires (UR892), INRA
Sabine Riffault: Unité de Virologie et Immunologie Moleculaires (UR892), INRA
Qin Yu: Infection Innovative Medicines Unit, AstraZeneca R&D Boston, 35 Gatehouse Drive, Waltham, Massachusetts 02451, USA
Marie Galloux: Unité de Virologie et Immunologie Moleculaires (UR892), INRA
Elyanne Gault: Physiopathologie et diagnostic des infections microbiennes, EA3647—EPIM, UFR des Sciences de la Santé Simone Veil—UVSQ
Jean-François Eléouët: Unité de Virologie et Immunologie Moleculaires (UR892), INRA

Nature Communications, 2014, vol. 5, issue 1, 1-10

Abstract: Abstract Respiratory syncytial virus (RSV) is the most important cause of severe lower-respiratory tract disease in calves and young children, yet no human vaccine nor efficient curative treatments are available. Here we describe a recombinant human RSV reverse genetics system in which the red fluorescent protein (mCherry) or the firefly luciferase (Luc) genes are inserted into the RSV genome. Expression of mCherry and Luc are correlated with infection rate, allowing the monitoring of RSV multiplication in cell culture. Replication of the Luc-encoding virus in living mice can be visualized by bioluminescent imaging, bioluminescence being detected in the snout and lungs of infected mice after nasal inoculation. We propose that these recombinant viruses are convenient and valuable tools for screening of compounds active against RSV, and can be used as an extremely sensitive readout for studying effects of antiviral therapeutics in living mice.

Date: 2014
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DOI: 10.1038/ncomms6104

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