Covalently linked hopanoid-lipid A improves outer-membrane resistance of a Bradyrhizobium symbiont of legumes

Alba Silipo (), Giuseppe Vitiello, Djamel Gully, Luisa Sturiale, Clémence Chaintreuil, Joel Fardoux, Daniel Gargani, Hae-In Lee, Gargi Kulkarni, Nicolas Busset, Roberta Marchetti, Angelo Palmigiano, Herman Moll, Regina Engel, Rosa Lanzetta, Luigi Paduano, Michelangelo Parrilli, Woo-Suk Chang, Otto Holst, Dianne K. Newman, Domenico Garozzo, Gerardino D’Errico, Eric Giraud () and Antonio Molinaro ()
Additional contact information
Alba Silipo: Complesso Universitario Monte Sant’Angelo, Università di Napoli Federico II
Giuseppe Vitiello: Complesso Universitario Monte Sant’Angelo, Università di Napoli Federico II
Djamel Gully: IRD, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), UMR IRD/SupAgro/INRA/UM2/CIRAD, TA-A82/J, Campus de Baillarguet
Luisa Sturiale: CNR–Istituto per i Polimeri, Compositi e Biomateriali IPCB, Unità di Catania Via P. Gaifami 18
Clémence Chaintreuil: IRD, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), UMR IRD/SupAgro/INRA/UM2/CIRAD, TA-A82/J, Campus de Baillarguet
Joel Fardoux: IRD, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), UMR IRD/SupAgro/INRA/UM2/CIRAD, TA-A82/J, Campus de Baillarguet
Daniel Gargani: CIRAD, UMR BGPI
Hae-In Lee: University of Texas
Gargi Kulkarni: California Institute of Technology and Howards Hughes Medical Institute
Nicolas Busset: IRD, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), UMR IRD/SupAgro/INRA/UM2/CIRAD, TA-A82/J, Campus de Baillarguet
Roberta Marchetti: Complesso Universitario Monte Sant’Angelo, Università di Napoli Federico II
Angelo Palmigiano: CNR–Istituto per i Polimeri, Compositi e Biomateriali IPCB, Unità di Catania Via P. Gaifami 18
Herman Moll: Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 4a/c
Regina Engel: Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 4a/c
Rosa Lanzetta: Complesso Universitario Monte Sant’Angelo, Università di Napoli Federico II
Luigi Paduano: Complesso Universitario Monte Sant’Angelo, Università di Napoli Federico II
Michelangelo Parrilli: Complesso Universitario Monte Sant’Angelo, Università di Napoli Federico II
Woo-Suk Chang: University of Texas
Otto Holst: Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 4a/c
Dianne K. Newman: California Institute of Technology and Howards Hughes Medical Institute
Domenico Garozzo: CNR–Istituto per i Polimeri, Compositi e Biomateriali IPCB, Unità di Catania Via P. Gaifami 18
Gerardino D’Errico: Complesso Universitario Monte Sant’Angelo, Università di Napoli Federico II
Eric Giraud: IRD, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), UMR IRD/SupAgro/INRA/UM2/CIRAD, TA-A82/J, Campus de Baillarguet
Antonio Molinaro: Complesso Universitario Monte Sant’Angelo, Università di Napoli Federico II

Nature Communications, 2014, vol. 5, issue 1, 1-11

Abstract: Abstract Lipopolysaccharides (LPSs) are major components of the outer membrane of Gram-negative bacteria and are essential for their growth and survival. They act as a structural barrier and play an important role in the interaction with eukaryotic hosts. Here we demonstrate that a photosynthetic Bradyrhizobium strain, symbiont of Aeschynomene legumes, synthesizes a unique LPS bearing a hopanoid covalently attached to lipid A. Biophysical analyses of reconstituted liposomes indicate that this hopanoid-lipid A structure reinforces the stability and rigidity of the outer membrane. In addition, the bacterium produces other hopanoid molecules not linked to LPS. A hopanoid-deficient strain, lacking a squalene hopene cyclase, displays increased sensitivity to stressful conditions and reduced ability to survive intracellularly in the host plant. This unusual combination of hopanoid and LPS molecules may represent an adaptation to optimize bacterial survival in both free-living and symbiotic states.

Date: 2014
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DOI: 10.1038/ncomms6106

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