Unravelling the mechanism of non-ribosomal peptide synthesis by cyclodipeptide synthases

Mireille Moutiez, Emmanuelle Schmitt, Jérôme Seguin, Robert Thai, Emmanuel Favry, Pascal Belin, Yves Mechulam () and Muriel Gondry ()
Additional contact information
Mireille Moutiez: Service d’Ingénierie Moléculaire des Protéines, iBiTec-S, Commissariat à l’Energie Atomique et aux Energies Alternatives (CEA)
Emmanuelle Schmitt: Laboratoire de Biochimie, Unité mixte de Recherche 7654, Ecole Polytechnique, Centre National de la Recherche Scientifique
Jérôme Seguin: Service d’Ingénierie Moléculaire des Protéines, iBiTec-S, Commissariat à l’Energie Atomique et aux Energies Alternatives (CEA)
Robert Thai: Service d’Ingénierie Moléculaire des Protéines, iBiTec-S, Commissariat à l’Energie Atomique et aux Energies Alternatives (CEA)
Emmanuel Favry: Service d’Ingénierie Moléculaire des Protéines, iBiTec-S, Commissariat à l’Energie Atomique et aux Energies Alternatives (CEA)
Pascal Belin: Service d’Ingénierie Moléculaire des Protéines, iBiTec-S, Commissariat à l’Energie Atomique et aux Energies Alternatives (CEA)
Yves Mechulam: Laboratoire de Biochimie, Unité mixte de Recherche 7654, Ecole Polytechnique, Centre National de la Recherche Scientifique
Muriel Gondry: Service d’Ingénierie Moléculaire des Protéines, iBiTec-S, Commissariat à l’Energie Atomique et aux Energies Alternatives (CEA)

Nature Communications, 2014, vol. 5, issue 1, 1-7

Abstract: Abstract Cyclodipeptide synthases form cyclodipeptides from two aminoacyl transfer RNAs. They use a ping-pong mechanism that begins with transfer of the aminoacyl moiety of the first aminoacyl tRNA onto a conserved serine, yielding an aminoacyl enzyme. Combining X-ray crystallography, site-directed mutagenesis and affinity labelling of the cyclodipeptide synthase AlbC, we demonstrate that the covalent intermediate reacts with the aminoacyl moiety of the second aminoacyl tRNA, forming a dipeptidyl enzyme, and identify the aminoacyl-binding sites of the aminoacyl tRNAs.

Date: 2014
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/ncomms6141 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6141

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms6141

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().