A long Stokes shift red fluorescent Ca2+ indicator protein for two-photon and ratiometric imaging

Jiahui Wu, Ahmed S. Abdelfattah, Loïs S. Miraucourt, Elena Kutsarova, Araya Ruangkittisakul, Hang Zhou, Klaus Ballanyi, Geoffrey Wicks, Mikhail Drobizhev, Aleksander Rebane, Edward S. Ruthazer and Robert E. Campbell ()
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Jiahui Wu: University of Alberta
Ahmed S. Abdelfattah: University of Alberta
Loïs S. Miraucourt: Montreal Neurological Institute, Neuroengineering Program, McGill University
Elena Kutsarova: Montreal Neurological Institute, Neuroengineering Program, McGill University
Araya Ruangkittisakul: University of Alberta
Hang Zhou: University of Alberta
Klaus Ballanyi: University of Alberta
Geoffrey Wicks: Montana State University
Mikhail Drobizhev: Montana State University
Aleksander Rebane: Montana State University
Edward S. Ruthazer: Montreal Neurological Institute, Neuroengineering Program, McGill University
Robert E. Campbell: University of Alberta

Nature Communications, 2014, vol. 5, issue 1, 1-11

Abstract: Abstract The introduction of calcium ion (Ca2+) indicators based on red fluorescent proteins (RFPs) has created new opportunities for multicolour visualization of intracellular Ca2+ dynamics. However, one drawback of these indicators is that they have optimal two-photon excitation outside the near-infrared window (650–1,000 nm) where tissue is most transparent to light. To address this shortcoming, we developed a long Stokes shift RFP-based Ca2+ indicator, REX-GECO1, with optimal two-photon excitation at

Date: 2014
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DOI: 10.1038/ncomms6262

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