MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane

Yoshinori Sato, Kenji Hayashi, Yoshiko Amano, Mikiko Takahashi, Shigenobu Yonemura, Ikuko Hayashi, Hiroko Hirose, Shigeo Ohno and Atsushi Suzuki ()
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Yoshinori Sato: Molecular Cellular Biology Laboratory, Yokohama City University, Graduate School of Medical Science
Kenji Hayashi: Molecular Cellular Biology Laboratory, Yokohama City University, Graduate School of Medical Science
Yoshiko Amano: Molecular Cellular Biology Laboratory, Yokohama City University, Graduate School of Medical Science
Mikiko Takahashi: Faculty of Pharmaceutical Sciences, Teikyo Heisei University
Shigenobu Yonemura: Electron Microscope Laboratory, RIKEN Center for Developmental Biology
Ikuko Hayashi: Molecular Medical Bioscience Laboratory, Yokohama City University, Graduate School of Medical Life Science
Hiroko Hirose: Yokohama City University, Graduate School of Medical Science
Shigeo Ohno: Yokohama City University, Graduate School of Medical Science
Atsushi Suzuki: Molecular Cellular Biology Laboratory, Yokohama City University, Graduate School of Medical Science

Nature Communications, 2014, vol. 5, issue 1, 1-14

Abstract: Abstract Recent studies have revealed the presence of a microtubule subpopulation called Golgi-derived microtubules that support Golgi ribbon formation, which is required for maintaining polarized cell migration. CLASPs and AKAP450/CG-NAP are involved in their formation, but the underlying molecular mechanisms remain unclear. Here, we find that the microtubule-crosslinking protein, MTCL1, is recruited to the Golgi membranes through interactions with CLASPs and AKAP450/CG-NAP, and promotes microtubule growth from the Golgi membrane. Correspondingly, MTCL1 knockdown specifically impairs the formation of the stable perinuclear microtubule network to which the Golgi ribbon tethers and extends. Rescue experiments demonstrate that besides its crosslinking activity mediated by the N-terminal microtubule-binding region, the C-terminal microtubule-binding region plays essential roles in these MTCL1 functions through a novel microtubule-stabilizing activity. These results suggest that MTCL1 cooperates with CLASPs and AKAP450/CG-NAP in the formation of the Golgi-derived microtubules, and mediates their development into a stable microtubule network.

Date: 2014
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DOI: 10.1038/ncomms6266

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