Amino- and carboxyl-terminal domains of Filamin-A interact with CRMP1 to mediate Sema3A signalling

Fumio Nakamura (), Kosuke Kumeta, Tomonobu Hida, Toshinari Isono, Yuichi Nakayama, Emiko Kuramata-Matsuoka, Naoya Yamashita, Yutaka Uchida, Ken-ichi Ogura, Keiko Gengyo-Ando, Shohei Mitani, Toshio Ogino and Yoshio Goshima ()
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Fumio Nakamura: Yokohama City University Graduate School of Medicine
Kosuke Kumeta: Yokohama City University Graduate School of Medicine
Tomonobu Hida: Yokohama City University Graduate School of Medicine
Toshinari Isono: Yokohama City University Graduate School of Medicine
Yuichi Nakayama: Yokohama City University Graduate School of Medicine
Emiko Kuramata-Matsuoka: Yokohama City University Graduate School of Medicine
Naoya Yamashita: Yokohama City University Graduate School of Medicine
Yutaka Uchida: Yokohama City University Graduate School of Medicine
Ken-ichi Ogura: Yokohama City University Graduate School of Medicine
Keiko Gengyo-Ando: Tokyo Women’s Medical University School of Medicine
Shohei Mitani: Tokyo Women’s Medical University School of Medicine
Toshio Ogino: Yokohama National University
Yoshio Goshima: Yokohama City University Graduate School of Medicine

Nature Communications, 2014, vol. 5, issue 1, 1-14

Abstract: Abstract Reorganization of the actin cytoskeleton is an early cellular response to various extracellular signals. Sema3A, a repulsive axon guidance molecule, induces the reorganization of actin cytoskeleton in the growth cones. Collapsin response mediator protein 1 (CRMP1) mediates the intracellular Sema3A signalling through its Ser522 phosphorylation. Here we show that UNC-33, CRMP1 C. elegans homologue, interacts with FLN-1, an actin-binding Filamin-A orthologue. In nematodes, this interaction participates in the projection of DD/VD motor neurons. CRMP1 binds both the actin-binding domain and the last immunoglobulin-like repeat of Filamin-A. The alanine mutants of Filamin-A or CRMP1 in their interacting residues suppress the Sema3A repulsion in neurons. Conversely, a phosphor-mimicking mutant CRMP1(Ser522Asp) enhances the Sema3A response. Atomic-force microscopy analysis reveals that the V-shaped Filamin-A changes to a condensed form with CRMP1(Ser522Asp). CRMP1(Ser522Asp) weakens the F-actin gelation crosslinked by Filamin-A. Thus, phosphorylated CRMP1 may remove Filamin-A from the actin cytoskeleton to facilitate its remodelling.

Date: 2014
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DOI: 10.1038/ncomms6325

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