A haploid genetics toolbox for Arabidopsis thaliana

Maruthachalam Ravi (), Mohan Prem Anand Marimuthu, Ek Han Tan, Shamoni Maheshwari, Isabelle M. Henry, Brenda Marin-Rodriguez, Guillaume Urtecho, Jie Tan, Kristina Thornhill, Fan Zhu, Aneesh Panoli, Venkatesan Sundaresan, Anne B. Britt, Luca Comai and Simon W. L. Chan
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Maruthachalam Ravi: University of California
Mohan Prem Anand Marimuthu: University of California
Ek Han Tan: University of California
Shamoni Maheshwari: University of California
Isabelle M. Henry: University of California
Brenda Marin-Rodriguez: University of California
Guillaume Urtecho: University of California
Jie Tan: University of California
Kristina Thornhill: University of California
Fan Zhu: University of California
Aneesh Panoli: University of California
Venkatesan Sundaresan: University of California
Anne B. Britt: University of California
Luca Comai: University of California
Simon W. L. Chan: University of California

Nature Communications, 2014, vol. 5, issue 1, 1-8

Abstract: Abstract Genetic analysis in haploids provides unconventional yet powerful advantages not available in diploid organisms. In Arabidopsis thaliana, haploids can be generated through seeds by crossing a wild-type strain to a transgenic strain with altered centromeres. Here we report the development of an improved haploid inducer (HI) strain, SeedGFP-HI, that aids selection of haploid seeds prior to germination. We also show that haploids can be used as a tool to accelerate a variety of genetic analyses, specifically pyramiding multiple mutant combinations, forward mutagenesis screens, scaling down a tetraploid to lower ploidy levels and swapping of nuclear and cytoplasmic genomes. Furthermore, the A. thaliana HI can be used to produce haploids from a related species A. suecica and generate homozygous mutant plants from strong maternal gametophyte lethal alleles, which is not possible via conventional diploid genetics. Taken together, our results demonstrate the utility and power of haploid genetics in A. thaliana.

Date: 2014
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DOI: 10.1038/ncomms6334

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