Omega-1 knockdown in Schistosoma mansoni eggs by lentivirus transduction reduces granuloma size in vivo

Jana Hagen, Neil D. Young, Alison L. Every, Charles N. Pagel, Corinna Schnoeller, Jean-Pierre Y. Scheerlinck, Robin B. Gasser () and Bernd H. Kalinna ()
Additional contact information
Jana Hagen: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne
Neil D. Young: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne
Alison L. Every: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne
Charles N. Pagel: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne
Corinna Schnoeller: Imperial College London
Jean-Pierre Y. Scheerlinck: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne
Robin B. Gasser: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne
Bernd H. Kalinna: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne

Nature Communications, 2014, vol. 5, issue 1, 1-9

Abstract: Abstract Schistosomiasis, one of the most important neglected tropical diseases worldwide, is caused by flatworms (blood flukes or schistosomes) that live in the bloodstream of humans. The hepatointestinal form of this debilitating disease results from a chronic infection with Schistosoma mansoni or Schistosoma japonicum. No vaccine is available to prevent schistosomiasis, and treatment relies predominantly on the use of a single drug, praziquantel. In spite of considerable research effort over the years, very little is known about the complex in vivo events that lead to granuloma formation and other pathological changes during infection. Here we use, for the first time, a lentivirus-based transduction system to deliver microRNA-adapted short hairpin RNAs (shRNAmirs) into the parasite to silence and explore selected protein-encoding genes of S. mansoni implicated in the disease process. This gene-silencing system has potential to be used for functional genomic–phenomic studies of a range of socioeconomically important pathogens.

Date: 2014
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms6375 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6375

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms6375

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().