TALEN and CRISPR/Cas9-mediated genome editing in the early-branching metazoan Nematostella vectensis

Aissam Ikmi, Sean A. McKinney, Kym M. Delventhal and Matthew C. Gibson ()
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Aissam Ikmi: Stowers Institute for Medical Research
Sean A. McKinney: Stowers Institute for Medical Research
Kym M. Delventhal: Stowers Institute for Medical Research
Matthew C. Gibson: Stowers Institute for Medical Research

Nature Communications, 2014, vol. 5, issue 1, 1-8

Abstract: Abstract Non-bilaterian phyla represent key lineages for exploring the evolutionary history of early animals. However, despite an increasing number of sequenced genomes from early-branching metazoans, efficient and reproducible methodologies for analysis of gene function remain a major challenge. Here we report the utilization of the TALEN and CRISPR/Cas9 systems to induce targeted mutations and homologous recombination-mediated transgenesis in the sea anemone Nematostella vectensis. We also present a new method to isolate genetically modified animals using engineered selection cassettes introduced by homologous recombination. Taken together, these methods will permit sophisticated gain- and loss-of-function analyses in Nematostella and perhaps other early metazoan species that allow for zygotic injection.

Date: 2014
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DOI: 10.1038/ncomms6486

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