Singular localization of sodium channel β4 subunit in unmyelinated fibres and its role in the striatum

Haruko Miyazaki, Fumitaka Oyama, Ritsuko Inoue, Toshihiko Aosaki, Takaya Abe, Hiroshi Kiyonari, Yoshihiro Kino, Masaru Kurosawa, Jun Shimizu, Ikuo Ogiwara, Kazuhiro Yamakawa, Yoshinori Koshimizu, Fumino Fujiyama, Takeshi Kaneko, Hideaki Shimizu, Katsuhiro Nagatomo, Katsuya Yamada, Tomomi Shimogori, Nobutaka Hattori, Masami Miura and Nobuyuki Nukina ()
Additional contact information
Haruko Miyazaki: Juntendo University Graduate School of Medicine
Fumitaka Oyama: Laboratory for Structural Neuropathology, RIKEN Brain Science Institute
Ritsuko Inoue: Neurophysiology Research Group, Tokyo Metropolitan Institute of Gerontology
Toshihiko Aosaki: Neurophysiology Research Group, Tokyo Metropolitan Institute of Gerontology
Takaya Abe: Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology
Hiroshi Kiyonari: Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology
Yoshihiro Kino: Juntendo University Graduate School of Medicine
Masaru Kurosawa: Juntendo University Graduate School of Medicine
Jun Shimizu: University of Tokyo
Ikuo Ogiwara: Laboratory for Neurogenetics, RIKEN Brain Science Institute
Kazuhiro Yamakawa: Laboratory for Neurogenetics, RIKEN Brain Science Institute
Yoshinori Koshimizu: Graduate School of Medicine, Kyoto University
Fumino Fujiyama: CREST (Core Research for Evolutionary Science and Technology), JST
Takeshi Kaneko: Graduate School of Medicine, Kyoto University
Hideaki Shimizu: RIKEN Center for Life Science Technology
Katsuhiro Nagatomo: Hirosaki University School of Medicine
Katsuya Yamada: Hirosaki University School of Medicine
Tomomi Shimogori: Laboratory for Molecular Mechanisms of Thalamus Development, RIKEN Brain Science Institute
Nobutaka Hattori: Juntendo University Graduate School of Medicine
Masami Miura: Neurophysiology Research Group, Tokyo Metropolitan Institute of Gerontology
Nobuyuki Nukina: Juntendo University Graduate School of Medicine

Nature Communications, 2014, vol. 5, issue 1, 1-15

Abstract: Abstract Voltage-gated Na+ channel β-subunits are multifunctional molecules that modulate Na+ channel activity and regulate cell adhesion, migration and neurite outgrowth. β-subunits including β4 are known to be highly concentrated in the nodes of Ranvier and axon initial segments in myelinated axons. Here we show diffuse β4 localization in striatal projection fibres using transgenic mice that express fluorescent protein in those fibres. These axons are unmyelinated, forming large, inhibitory fibre bundles. Furthermore, we report β4 dimer expression in the mouse brain, with high levels of β4 dimers in the striatal projection fascicles, suggesting a specific role of β4 in those fibres. Scn4b-deficient mice show a resurgent Na+ current reduction, decreased repetitive firing frequency in medium spiny neurons and increased failure rates of inhibitory postsynaptic currents evoked with repetitive stimulation, indicating an in vivo channel regulatory role of β4 in the striatum.

Date: 2014
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms6525 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6525

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms6525

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().