Regulation of Drosophila circadian rhythms by miRNA let-7 is mediated by a regulatory cycle

Wenfeng Chen, Zhenxing Liu, Tianjiao Li, Ruifeng Zhang, Yongbo Xue, Yang Zhong, Weiwei Bai, Dasen Zhou and Zhangwu Zhao ()
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Wenfeng Chen: College of Agronomy and Biotechnology, China Agricultural University
Zhenxing Liu: College of Agronomy and Biotechnology, China Agricultural University
Tianjiao Li: College of Agronomy and Biotechnology, China Agricultural University
Ruifeng Zhang: College of Agronomy and Biotechnology, China Agricultural University
Yongbo Xue: College of Agronomy and Biotechnology, China Agricultural University
Yang Zhong: College of Agronomy and Biotechnology, China Agricultural University
Weiwei Bai: College of Agronomy and Biotechnology, China Agricultural University
Dasen Zhou: College of Agronomy and Biotechnology, China Agricultural University
Zhangwu Zhao: College of Agronomy and Biotechnology, China Agricultural University

Nature Communications, 2014, vol. 5, issue 1, 1-13

Abstract: Abstract MicroRNA-mediated post-transcriptional regulations are increasingly recognized as important components of the circadian rhythm. Here we identify microRNA let-7, part of the Drosophila let-7-Complex, as a regulator of circadian rhythms mediated by a circadian regulatory cycle. Overexpression of let-7 in clock neurons lengthens circadian period and its deletion attenuates the morning activity peak as well as molecular oscillation. Let-7 regulates the circadian rhythm via repression of CLOCKWORK ORANGE (CWO). Conversely, upregulated cwo in cwo-expressing cells can rescue the phenotype of let-7-Complex overexpression. Moreover, circadian prothoracicotropic hormone (PTTH) and CLOCK-regulated 20-OH ecdysteroid signalling contribute to the circadian expression of let-7 through the 20-OH ecdysteroid receptor. Thus, we find a regulatory cycle involving PTTH, a direct target of CLOCK, and PTTH-driven miRNA let-7.

Date: 2014
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DOI: 10.1038/ncomms6549

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