Assembling programmable FRET-based photonic networks using designer DNA scaffolds

Susan Buckhout-White, Christopher M Spillmann, W. Russ Algar, Ani Khachatrian, Joseph S. Melinger, Ellen R. Goldman, Mario G. Ancona and Igor L. Medintz ()
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Susan Buckhout-White: Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory
Christopher M Spillmann: Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory
W. Russ Algar: Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory
Ani Khachatrian: Code 6800, U.S. Naval Research Laboratory
Joseph S. Melinger: Code 6800, U.S. Naval Research Laboratory
Ellen R. Goldman: Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory
Mario G. Ancona: Code 6800, U.S. Naval Research Laboratory
Igor L. Medintz: Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory

Nature Communications, 2014, vol. 5, issue 1, 1-16

Abstract: Abstract DNA demonstrates a remarkable capacity for creating designer nanostructures and devices. A growing number of these structures utilize Förster resonance energy transfer (FRET) as part of the device's functionality, readout or characterization, and, as device sophistication increases so do the concomitant FRET requirements. Here we create multi-dye FRET cascades and assess how well DNA can marshal organic dyes into nanoantennae that focus excitonic energy. We evaluate 36 increasingly complex designs including linear, bifurcated, Holliday junction, 8-arm star and dendrimers involving up to five different dyes engaging in four-consecutive FRET steps, while systematically varying fluorophore spacing by Förster distance (R0). Decreasing R0 while augmenting cross-sectional collection area with multiple donors significantly increases terminal exciton delivery efficiency within dendrimers compared with the first linear constructs. Förster modelling confirms that best results are obtained when there are multiple interacting FRET pathways rather than independent channels by which excitons travel from initial donor(s) to final acceptor.

Date: 2014
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DOI: 10.1038/ncomms6615

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