An ecdysone-responsive nuclear receptor regulates circadian rhythms in Drosophila

Shailesh Kumar, Dechun Chen, Christopher Jang, Alexandra Nall, Xiangzhong Zheng and Amita Sehgal ()
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Shailesh Kumar: University of Pennsylvania
Dechun Chen: University of Pennsylvania
Christopher Jang: University of Pennsylvania
Alexandra Nall: University of Pennsylvania
Xiangzhong Zheng: University of Pennsylvania
Amita Sehgal: University of Pennsylvania

Nature Communications, 2014, vol. 5, issue 1, 1-12

Abstract: Abstract Little is known about molecular links between circadian clocks and steroid hormone signalling, although both are important for normal physiology. Here we report a circadian function for a nuclear receptor, ecdysone-induced protein 75 (Eip75/E75), which we identified through a gain-of-function screen for circadian genes in Drosophila melanogaster. Overexpression or knockdown of E75 in clock neurons disrupts rest:activity rhythms and dampens molecular oscillations. E75 represses expression of the gene encoding the transcriptional activator, CLOCK (CLK), and may also affect circadian output. PER inhibits the activity of E75 on the Clk promoter, thereby providing a mechanism for a previously proposed de-repressor effect of PER on Clk transcription. The ecdysone receptor is also expressed in central clock cells and manipulations of its expression produce effects similar to those of E75 on circadian rhythms. We find that E75 protects rhythms under stressful conditions, suggesting a function for steroid signalling in the maintenance of circadian rhythms in Drosophila.

Date: 2014
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DOI: 10.1038/ncomms6697

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