Structured line illumination Raman microscopy

Kozue Watanabe, Almar F. Palonpon, Nicholas I. Smith, Liang-da Chiu, Atsushi Kasai, Hitoshi Hashimoto, Satoshi Kawata and Katsumasa Fujita ()
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Kozue Watanabe: Graduate School of Engineering, Osaka University
Almar F. Palonpon: Graduate School of Engineering, Osaka University
Nicholas I. Smith: Immunology Frontier Research Center, Osaka University
Liang-da Chiu: Graduate School of Engineering, Osaka University
Atsushi Kasai: Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University
Hitoshi Hashimoto: Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University
Satoshi Kawata: Graduate School of Engineering, Osaka University
Katsumasa Fujita: Graduate School of Engineering, Osaka University

Nature Communications, 2015, vol. 6, issue 1, 1-8

Abstract: Abstract In the last couple of decades, the spatial resolution in optical microscopy has increased to unprecedented levels by exploiting the fluorescence properties of the probe. At about the same time, Raman imaging techniques have emerged as a way to image inherent chemical information in a sample without using fluorescent probes. However, in many applications, the achievable resolution is limited to about half the wavelength of excitation light. Here we report the use of structured illumination to increase the spatial resolution of label-free spontaneous Raman microscopy, generating highly detailed spatial contrast from the ensemble of molecular information in the sample. Using structured line illumination in slit-scanning Raman microscopy, we demonstrate a marked improvement in spatial resolution and show the applicability to a range of samples, including both biological and inorganic chemical component mapping. This technique is expected to contribute towards greater understanding of chemical component distributions in organic and inorganic materials.

Date: 2015
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DOI: 10.1038/ncomms10095

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