PAM multiplicity marks genomic target sites as inhibitory to CRISPR-Cas9 editing

Abba Malina, Christopher J. F. Cameron, Francis Robert, Mathieu Blanchette, Josée Dostie and Jerry Pelletier ()
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Abba Malina: McGill University
Christopher J. F. Cameron: McGill Centre for Bioinformatics and School of Computer Science, McGill University
Francis Robert: McGill University
Mathieu Blanchette: McGill Centre for Bioinformatics and School of Computer Science, McGill University
Josée Dostie: McGill University
Jerry Pelletier: McGill University

Nature Communications, 2015, vol. 6, issue 1, 1-6

Abstract: Abstract In CRISPR-Cas9 genome editing, the underlying principles for selecting guide RNA (gRNA) sequences that would ensure for efficient target site modification remain poorly understood. Here we show that target sites harbouring multiple protospacer adjacent motifs (PAMs) are refractory to Cas9-mediated repair in situ. Thus we refine which substrates should be avoided in gRNA design, implicating PAM density as a novel sequence-specific feature that inhibits in vivo Cas9-driven DNA modification.

Date: 2015
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms10124

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DOI: 10.1038/ncomms10124

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