Identification of a subnuclear body involved in sequence-specific cytokine RNA processing

Sungwook Lee, Taeyun A. Lee, Eunhye Lee, Sujin Kang, Areum Park, Seung Won Kim, Hyo Jin Park, Je-Hyun Yoon, Sang-Jun Ha, Taesun Park, Ju-Seog Lee, Jae Hee Cheon and Boyoun Park ()
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Sungwook Lee: College of Life Science and Biotechnology, Yonsei University
Taeyun A. Lee: College of Life Science and Biotechnology, Yonsei University
Eunhye Lee: College of Life Science and Biotechnology, Yonsei University
Sujin Kang: College of Life Science and Biotechnology, Yonsei University
Areum Park: College of Life Science and Biotechnology, Yonsei University
Seung Won Kim: Yonsei University College of Medicine
Hyo Jin Park: College of Life Science and Biotechnology, Yonsei University
Je-Hyun Yoon: Laboratory of Genetics, National Institute on Aging-Intramural Research Program, NIH
Sang-Jun Ha: College of Life Science and Biotechnology, Yonsei University
Taesun Park: College of Human Ecology, Yonsei University
Ju-Seog Lee: University of Texas MD Anderson Cancer Center
Jae Hee Cheon: Yonsei University College of Medicine
Boyoun Park: College of Life Science and Biotechnology, Yonsei University

Nature Communications, 2015, vol. 6, issue 1, 1-14

Abstract: Abstract Processing of interleukin RNAs must be tightly controlled during the immune response. Here we report that a subnuclear body called the interleukin-6 and -10 splicing activating compartment (InSAC) is a nuclear site of cytokine RNA production and stability. Tat-activating regulatory DNA-binding protein-43 (TDP-43) acts as an InSAC scaffold that selectively associates with IL-6 and IL-10 RNAs in a sequence-specific manner. TDP-43 also recruits key spliceosomal components from Cajal bodies. LPS induces posttranslational modifications of TDP-43; in particular, TDP-43 ubiquitination provides a driving force for InSAC formation. As a consequence, in vivo depletion of TDP-43 leads to a dramatic reduction in the RNA processing and the protein levels of IL-6 in serum. Collectively, our findings highlight the importance of TDP-43-mediated InSAC biogenesis in immune regulation.

Date: 2015
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DOI: 10.1038/ncomms6791

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