A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform

Howon Lee, Hyoki Kim, Sungsik Kim, Taehoon Ryu, Hwangbeom Kim, Duhee Bang () and Sunghoon Kwon ()
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Howon Lee: Institutes of Entrepreneurial BioConvergence, Seoul National University
Hyoki Kim: Celemics Inc., 371-17, Gasan-dong, Geumcheon-gu
Sungsik Kim: Institutes of Entrepreneurial BioConvergence, Seoul National University
Taehoon Ryu: Institutes of Entrepreneurial BioConvergence, Seoul National University
Hwangbeom Kim: University of California, Los Angeles
Duhee Bang: Yonsei University
Sunghoon Kwon: Institutes of Entrepreneurial BioConvergence, Seoul National University

Nature Communications, 2015, vol. 6, issue 1, 1-7

Abstract: Abstract Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. DNA clones on next-generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from high-density NGS platforms. Here we propose an enabling technology called ‘Sniper Cloning’ that enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for the full utilization of DNA clones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, Sniper Cloning is a week-long process that produces 5,188 error-free synthetic DNAs in a single run of NGS with a single microarray DNA pool. We believe that this technology has potential as a universal tool for DNA writing in biological sciences.

Date: 2015
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DOI: 10.1038/ncomms7073

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