Capture Hi-C identifies the chromatin interactome of colorectal cancer risk loci

Roland Jäger, Gabriele Migliorini, Marc Henrion, Radhika Kandaswamy, Helen E. Speedy, Andreas Heindl, Nicola Whiffin, Maria J. Carnicer, Laura Broome, Nicola Dryden, Takashi Nagano, Stefan Schoenfelder, Martin Enge, Yinyin Yuan, Jussi Taipale, Peter Fraser, Olivia Fletcher and Richard S. Houlston ()
Additional contact information
Roland Jäger: The Institute of Cancer Research
Gabriele Migliorini: The Institute of Cancer Research
Marc Henrion: The Institute of Cancer Research
Radhika Kandaswamy: The Institute of Cancer Research
Helen E. Speedy: The Institute of Cancer Research
Andreas Heindl: The Institute of Cancer Research
Nicola Whiffin: The Institute of Cancer Research
Maria J. Carnicer: Haemato-Oncology Research Unit, The Institute of Cancer Research
Laura Broome: Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research
Nicola Dryden: Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research
Takashi Nagano: Nuclear Dynamics Programme, The Babraham Institute
Stefan Schoenfelder: Nuclear Dynamics Programme, The Babraham Institute
Martin Enge: Science for Life Laboratory, Karolinska Institutet
Yinyin Yuan: The Institute of Cancer Research
Jussi Taipale: Science for Life Laboratory, Karolinska Institutet
Peter Fraser: Nuclear Dynamics Programme, The Babraham Institute
Olivia Fletcher: Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research
Richard S. Houlston: The Institute of Cancer Research

Nature Communications, 2015, vol. 6, issue 1, 1-9

Abstract: Abstract Multiple regulatory elements distant from their targets on the linear genome can influence the expression of a single gene through chromatin looping. Chromosome conformation capture implemented in Hi-C allows for genome-wide agnostic characterization of chromatin contacts. However, detection of functional enhancer–promoter interactions is precluded by its effective resolution that is determined by both restriction fragmentation and sensitivity of the experiment. Here we develop a capture Hi-C (cHi-C) approach to allow an agnostic characterization of these physical interactions on a genome-wide scale. Single-nucleotide polymorphisms associated with complex diseases often reside within regulatory elements and exert effects through long-range regulation of gene expression. Applying this cHi-C approach to 14 colorectal cancer risk loci allows us to identify key long-range chromatin interactions in cis and trans involving these loci.

Date: 2015
References: Add references at CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.nature.com/articles/ncomms7178 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7178

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms7178

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().