Multi-spectral fluorescent reporter influenza viruses (Color-flu) as powerful tools for in vivo studies

Satoshi Fukuyama, Hiroaki Katsura, Dongming Zhao, Makoto Ozawa, Tomomi Ando, Jason E. Shoemaker, Izumi Ishikawa, Shinya Yamada, Gabriele Neumann, Shinji Watanabe, Hiroaki Kitano and Yoshihiro Kawaoka ()
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Satoshi Fukuyama: Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
Hiroaki Katsura: Institute of Medical Science, University of Tokyo
Dongming Zhao: Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
Makoto Ozawa: Laboratory of Animal Hygiene, Joint Faculty of Veterinary Medicine, Kagoshima University
Tomomi Ando: Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
Jason E. Shoemaker: Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
Izumi Ishikawa: Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
Shinya Yamada: Institute of Medical Science, University of Tokyo
Gabriele Neumann: School of Veterinary Medicine, University of Wisconsin-Madison
Shinji Watanabe: Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
Hiroaki Kitano: Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
Yoshihiro Kawaoka: Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)

Nature Communications, 2015, vol. 6, issue 1, 1-8

Abstract: Abstract Seasonal influenza A viruses cause annual epidemics of respiratory disease; highly pathogenic avian H5N1 and the recently emerged H7N9 viruses cause severe infections in humans, often with fatal outcomes. Although numerous studies have addressed the pathogenicity of influenza viruses, influenza pathogenesis remains incompletely understood. Here we generate influenza viruses expressing fluorescent proteins of different colours (‘Color-flu’ viruses) to facilitate the study of viral infection in in vivo models. On adaptation to mice, stable expression of the fluorescent proteins in infected animals allows their detection by different types of microscopy and by flow cytometry. We use this system to analyse the progression of viral spread in mouse lungs, for live imaging of virus-infected cells, and for differential gene expression studies in virus antigen-positive and virus antigen-negative live cells in the lungs of Color-flu-infected mice. Collectively, Color-flu viruses are powerful tools to analyse virus infections at the cellular level in vivo to better understand influenza pathogenesis.

Date: 2015
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DOI: 10.1038/ncomms7600

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