Metal ion-directed dynamic splicing of DNA through global conformational change by intramolecular complexation

Toshihiro Ihara (), Hiroyuki Ohura, Chisato Shirahama, Tomohiro Furuzono, Hiroshi Shimada, Hirotaka Matsuura and Yusuke Kitamura
Additional contact information
Toshihiro Ihara: Graduate School of Science and Technology, Kumamoto University
Hiroyuki Ohura: Graduate School of Science and Technology, Kumamoto University
Chisato Shirahama: Graduate School of Science and Technology, Kumamoto University
Tomohiro Furuzono: Graduate School of Science and Technology, Kumamoto University
Hiroshi Shimada: Graduate School of Science and Technology, Kumamoto University
Hirotaka Matsuura: Graduate School of Science and Technology, Kumamoto University
Yusuke Kitamura: Graduate School of Science and Technology, Kumamoto University

Nature Communications, 2015, vol. 6, issue 1, 1-8

Abstract: Abstract Chemically engineered DNAs—in which global conformation can be modulated in response to specific stimuli—could be allosteric functional DNAs themselves or work as a modulator of the functional nucleic acids such as DNAzymes and aptamers. Here, we show that two terpyridines built in the DNA backbone form a stable intramolecular 1:2 complex, [M(terpy)2]2+, with divalent transition metal ions. Upon complexation, the DNA conjugates adopt a Ω-shape structure, in which two distal sequences located outside the terpyridines connect with each other to form a continuous segment with a specific structure or sequence. Such a DNA structure is globally controlled by local metal complexation events that can be rationally designed based on general coordination chemistry. This method is regarded as metal ion-directed dynamic sequence edition or DNA splicing. DNAzymes with peroxidase-like activity can thus be regulated by several transition metal ions through sequence edition techniques based on the Ω-motif.

Date: 2015
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms7640 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7640

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms7640

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().