Arabidopsis uses two gluconeogenic gateways for organic acids to fuel seedling establishment

Peter J. Eastmond, Holly M. Astley, Kate Parsley, Sylvain Aubry, Ben P. Williams, Guillaume N. Menard, Christian P. Craddock, Adriano Nunes-Nesi, Alisdair R. Fernie and Julian M. Hibberd ()
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Peter J. Eastmond: Rothamsted Research, West Common
Holly M. Astley: University of Cambridge, Downing Street, Cambridge CB2 3EA, UK
Kate Parsley: University of Cambridge, Downing Street, Cambridge CB2 3EA, UK
Sylvain Aubry: University of Cambridge, Downing Street, Cambridge CB2 3EA, UK
Ben P. Williams: University of Cambridge, Downing Street, Cambridge CB2 3EA, UK
Guillaume N. Menard: Rothamsted Research, West Common
Christian P. Craddock: Rothamsted Research, West Common
Adriano Nunes-Nesi: Max-Planck-Insitüt für Molekulare Pflanzenphysiologie
Alisdair R. Fernie: Max-Planck-Insitüt für Molekulare Pflanzenphysiologie
Julian M. Hibberd: University of Cambridge, Downing Street, Cambridge CB2 3EA, UK

Nature Communications, 2015, vol. 6, issue 1, 1-8

Abstract: Abstract Gluconeogenesis is a fundamental metabolic process that allows organisms to make sugars from non-carbohydrate stores such as lipids and protein. In eukaryotes only one gluconeogenic route has been described from organic acid intermediates and this relies on the enzyme phosphoenolpyruvate carboxykinase (PCK). Here we show that two routes exist in Arabidopsis, and that the second uses pyruvate, orthophosphate dikinase (PPDK). Gluconeogenesis is critical to fuel the transition from seed to seedling. Arabidopsis pck1 and ppdk mutants are compromised in seed-storage reserve mobilization and seedling establishment. Radiolabelling studies show that PCK predominantly allows sugars to be made from dicarboxylic acids, which are products of lipid breakdown. However, PPDK also allows sugars to be made from pyruvate, which is a major product of protein breakdown. We propose that both routes have been evolutionarily conserved in plants because, while PCK expends less energy, PPDK is twice as efficient at recovering carbon from pyruvate.

Date: 2015
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DOI: 10.1038/ncomms7659

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