Systematic functional profiling of transcription factor networks in Cryptococcus neoformans

Kwang-Woo Jung, Dong-Hoon Yang, Shinae Maeng, Kyung-Tae Lee, Yee-Seul So, Joohyeon Hong, Jaeyoung Choi, Hyo-Jeong Byun, Hyelim Kim, Soohyun Bang, Min-Hee Song, Jang-Won Lee, Min Su Kim, Seo-Young Kim, Je-Hyun Ji, Goun Park, Hyojeong Kwon, Suyeon Cha, Gena Lee Meyers, Li Li Wang, Jooyoung Jang, Guilhem Janbon, Gloria Adedoyin, Taeyup Kim, Anna K. Averette, Joseph Heitman, Eunji Cheong, Yong-Hwan Lee, Yin-Won Lee and Yong-Sun Bahn ()
Additional contact information
Kwang-Woo Jung: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Dong-Hoon Yang: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Shinae Maeng: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Kyung-Tae Lee: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Yee-Seul So: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Joohyeon Hong: College of Life Science and Biotechnology, Yonsei University
Jaeyoung Choi: Center for Fungal Pathogenesis, Seoul National University
Hyo-Jeong Byun: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Hyelim Kim: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Soohyun Bang: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Min-Hee Song: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Jang-Won Lee: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Min Su Kim: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Seo-Young Kim: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Je-Hyun Ji: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Goun Park: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Hyojeong Kwon: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Suyeon Cha: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Gena Lee Meyers: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Li Li Wang: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Jooyoung Jang: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University
Guilhem Janbon: Unité Biologie et Pathogénicité Fongiques, Institut Pasteur
Gloria Adedoyin: Medicine, and Pharmacology and Cancer Biology, Duke University Medical Center
Taeyup Kim: Medicine, and Pharmacology and Cancer Biology, Duke University Medical Center
Anna K. Averette: Medicine, and Pharmacology and Cancer Biology, Duke University Medical Center
Joseph Heitman: Medicine, and Pharmacology and Cancer Biology, Duke University Medical Center
Eunji Cheong: College of Life Science and Biotechnology, Yonsei University
Yong-Hwan Lee: Center for Fungal Pathogenesis, Seoul National University
Yin-Won Lee: Center for Fungal Pathogenesis, Seoul National University
Yong-Sun Bahn: Center for Fungal Pathogenesis, College of Life Science and Biotechnology, Yonsei University

Nature Communications, 2015, vol. 6, issue 1, 1-14

Abstract: Abstract Cryptococcus neoformans causes life-threatening meningoencephalitis in humans, but its overall biological and pathogenic regulatory circuits remain elusive, particularly due to the presence of an evolutionarily divergent set of transcription factors (TFs). Here, we report the construction of a high-quality library of 322 signature-tagged gene-deletion strains for 155 putative TF genes previously predicted using the DNA-binding domain TF database, and examine their in vitro and in vivo phenotypic traits under 32 distinct growth conditions. At least one phenotypic trait is exhibited by 145 out of 155 TF mutants (93%) and ∼85% of them (132/155) are functionally characterized for the first time in this study. The genotypic and phenotypic data for each TF are available in the C. neoformans TF phenome database ( http://tf.cryptococcus.org ). In conclusion, our phenome-based functional analysis of the C. neoformans TF mutant library provides key insights into transcriptional networks of basidiomycetous fungi and human fungal pathogens.

Date: 2015
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/ncomms7757 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7757

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms7757

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().