Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells

Kyungjin Boo, Jinhyuk Bhin, Yoon Jeon, Joomyung Kim, Hi-Jai R. Shin, Jong-Eun Park, Kyeongkyu Kim, Chang Rok Kim, Hyonchol Jang, In-Hoo Kim, V. Narry Kim, Daehee Hwang (), Ho Lee () and Sung Hee Baek ()
Additional contact information
Kyungjin Boo: Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University
Jinhyuk Bhin: POSTECH
Yoon Jeon: Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center
Joomyung Kim: Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University
Hi-Jai R. Shin: Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University
Jong-Eun Park: Institute for Basic Science, School of Biological Sciences, Seoul National University
Kyeongkyu Kim: Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University
Chang Rok Kim: Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University
Hyonchol Jang: Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center
In-Hoo Kim: Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center
V. Narry Kim: Institute for Basic Science, School of Biological Sciences, Seoul National University
Daehee Hwang: POSTECH
Ho Lee: Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center
Sung Hee Baek: Creative Research Initiative Center for Chromatin Dynamics, School of Biological Sciences, Seoul National University

Nature Communications, 2015, vol. 6, issue 1, 1-16

Abstract: Abstract The actions of transcription factors, chromatin modifiers and noncoding RNAs are crucial for the programming of cell states. Although the importance of various epigenetic machineries for controlling pluripotency of embryonic stem (ES) cells has been previously studied, how chromatin modifiers cooperate with specific transcription factors still remains largely elusive. Here, we find that Pontin chromatin remodelling factor plays an essential role as a coactivator for Oct4 for maintenance of pluripotency in mouse ES cells. Genome-wide analyses reveal that Pontin and Oct4 share a substantial set of target genes involved in ES cell maintenance. Intriguingly, we find that the Oct4-dependent coactivator function of Pontin extends to the transcription of large intergenic noncoding RNAs (lincRNAs) and in particular linc1253, a lineage programme repressing lincRNA, is a Pontin-dependent Oct4 target lincRNA. Together, our findings demonstrate that the Oct4-Pontin module plays critical roles in the regulation of genes involved in ES cell fate determination.

Date: 2015
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DOI: 10.1038/ncomms7810

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