Single-band upconversion nanoprobes for multiplexed simultaneous in situ molecular mapping of cancer biomarkers

Lei Zhou, Rui Wang, Chi Yao, Xiaomin Li, Chengli Wang, Xiaoyan Zhang, Congjian Xu, Aijun Zeng, Dongyuan Zhao and Fan Zhang ()
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Lei Zhou: iChEm (Collaborative Innovation Center of Chemistry for Energy Materials), State Key Laboratory of Molecular Engineering of Polymers, Fudan University
Rui Wang: iChEm (Collaborative Innovation Center of Chemistry for Energy Materials), State Key Laboratory of Molecular Engineering of Polymers, Fudan University
Chi Yao: iChEm (Collaborative Innovation Center of Chemistry for Energy Materials), State Key Laboratory of Molecular Engineering of Polymers, Fudan University
Xiaomin Li: iChEm (Collaborative Innovation Center of Chemistry for Energy Materials), State Key Laboratory of Molecular Engineering of Polymers, Fudan University
Chengli Wang: iChEm (Collaborative Innovation Center of Chemistry for Energy Materials), State Key Laboratory of Molecular Engineering of Polymers, Fudan University
Xiaoyan Zhang: Obstetrics and Gynecology Hospital of Fudan University
Congjian Xu: Obstetrics and Gynecology Hospital of Fudan University
Aijun Zeng: Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences
Dongyuan Zhao: iChEm (Collaborative Innovation Center of Chemistry for Energy Materials), State Key Laboratory of Molecular Engineering of Polymers, Fudan University
Fan Zhang: iChEm (Collaborative Innovation Center of Chemistry for Energy Materials), State Key Laboratory of Molecular Engineering of Polymers, Fudan University

Nature Communications, 2015, vol. 6, issue 1, 1-12

Abstract: Abstract The identification of potential diagnostic markers and target molecules among the plethora of tumour oncoproteins for cancer diagnosis requires facile technology that is capable of quantitatively analysing multiple biomarkers in tumour cells and tissues. Diagnostic and prognostic classifications of human tumours are currently based on the western blotting and single-colour immunohistochemical methods that are not suitable for multiplexed detection. Herein, we report a general and novel method to prepare single-band upconversion nanoparticles with different colours. The expression levels of three biomarkers in breast cancer cells were determined using single-band upconversion nanoparticles, western blotting and immunohistochemical technologies with excellent correlation. Significantly, the application of antibody-conjugated single-band upconversion nanoparticle molecular profiling technology can achieve the multiplexed simultaneous in situ biodetection of biomarkers in breast cancer cells and tissue specimens and produce more accurate results for the simultaneous quantification of proteins present at low levels compared with classical immunohistochemical technology.

Date: 2015
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DOI: 10.1038/ncomms7938

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