In vivo model with targeted cAMP biosensor reveals changes in receptor–microdomain communication in cardiac disease

Julia U. Sprenger, Ruwan K. Perera, Julia H. Steinbrecher, Stephan E. Lehnart, Lars S. Maier, Gerd Hasenfuss and Viacheslav O. Nikolaev ()
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Julia U. Sprenger: Emmy Noether Group of the DFG, European Heart Research Institute Göttingen, University Medical Center Göttingen
Ruwan K. Perera: Emmy Noether Group of the DFG, European Heart Research Institute Göttingen, University Medical Center Göttingen
Julia H. Steinbrecher: Heart Research Center Göttingen, University Medical Center Göttingen, Georg August University
Stephan E. Lehnart: Heart Research Center Göttingen, University Medical Center Göttingen, Georg August University
Lars S. Maier: University Hospital Regensburg
Gerd Hasenfuss: Heart Research Center Göttingen, University Medical Center Göttingen, Georg August University
Viacheslav O. Nikolaev: Emmy Noether Group of the DFG, European Heart Research Institute Göttingen, University Medical Center Göttingen

Nature Communications, 2015, vol. 6, issue 1, 1-11

Abstract: Abstract 3′,5′-cyclic adenosine monophosphate (cAMP) is an ubiquitous second messenger that regulates physiological functions by acting in distinct subcellular microdomains. Although several targeted cAMP biosensors are developed and used in single cells, it is unclear whether such biosensors can be successfully applied in vivo, especially in the context of disease. Here, we describe a transgenic mouse model expressing a targeted cAMP sensor and analyse microdomain-specific second messenger dynamics in the vicinity of the sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA). We demonstrate the biocompatibility of this targeted sensor and its potential for real-time monitoring of compartmentalized cAMP signalling in adult cardiomyocytes isolated from a healthy mouse heart and from an in vivo cardiac disease model. In particular, we uncover the existence of a phosphodiesterase-dependent receptor–microdomain communication, which is affected in hypertrophy, resulting in reduced β-adrenergic receptor-cAMP signalling to SERCA.

Date: 2015
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DOI: 10.1038/ncomms7965

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