RNA structure determination by solid-state NMR spectroscopy

Alexander Marchanka (), Bernd Simon, Gerhard Althoff-Ospelt and Teresa Carlomagno ()
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Alexander Marchanka: Structural and Computational Biology Unit, European Molecular Biology Laboratory
Bernd Simon: Structural and Computational Biology Unit, European Molecular Biology Laboratory
Gerhard Althoff-Ospelt: Bruker BioSpin
Teresa Carlomagno: Structural and Computational Biology Unit, European Molecular Biology Laboratory

Nature Communications, 2015, vol. 6, issue 1, 1-7

Abstract: Abstract Knowledge of the RNA three-dimensional structure, either in isolation or as part of RNP complexes, is fundamental to understand the mechanism of numerous cellular processes. Because of its flexibility, RNA represents a challenge for crystallization, while the large size of cellular complexes brings solution-state NMR to its limits. Here, we demonstrate an alternative approach on the basis of solid-state NMR spectroscopy. We develop a suite of experiments and RNA labeling schemes and demonstrate for the first time that ssNMR can yield a RNA structure at high-resolution. This methodology allows structural analysis of segmentally labelled RNA stretches in high-molecular weight cellular machines—independent of their ability to crystallize— and opens the way to mechanistic studies of currently difficult-to-access RNA-protein assemblies.

Date: 2015
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8024

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DOI: 10.1038/ncomms8024

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