Clk post-transcriptional control denoises circadian transcription both temporally and spatially

Immanuel Lerner, Osnat Bartok, Victoria Wolfson, Jerome S. Menet, Uri Weissbein, Shaked Afik, Daniel Haimovich, Chen Gafni, Nir Friedman, Michael Rosbash and Sebastian Kadener ()
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Immanuel Lerner: Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University
Osnat Bartok: Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University
Victoria Wolfson: Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University
Jerome S. Menet: Howard Hughes Medical Institute, Brandeis University
Uri Weissbein: Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University
Shaked Afik: Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University
Daniel Haimovich: Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University
Chen Gafni: Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University
Nir Friedman: Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University
Michael Rosbash: Howard Hughes Medical Institute, Brandeis University
Sebastian Kadener: Silberman Institute of Life Sciences, Edmund J. Safra Campus, The Hebrew University

Nature Communications, 2015, vol. 6, issue 1, 1-12

Abstract: Abstract The transcription factor CLOCK (CLK) is essential for the development and maintenance of circadian rhythms in Drosophila. However, little is known about how CLK levels are controlled. Here we show that Clk mRNA is strongly regulated post-transcriptionally through its 3′ UTR. Flies expressing Clk transgenes without normal 3′ UTR exhibit variable CLK-driven transcription and circadian behaviour as well as ectopic expression of CLK-target genes in the brain. In these flies, the number of the key circadian neurons differs stochastically between individuals and within the two hemispheres of the same brain. Moreover, flies carrying Clk transgenes with deletions in the binding sites for the miRNA bantam have stochastic number of pacemaker neurons, suggesting that this miRNA mediates the deterministic expression of CLK. Overall our results demonstrate a key role of Clk post-transcriptional control in stabilizing circadian transcription, which is essential for proper development and maintenance of circadian rhythms in Drosophila.

Date: 2015
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DOI: 10.1038/ncomms8056

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