A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs

Tudor A. Fulga (), Elizabeth M. McNeill, Richard Binari, Julia Yelick, Alexandra Blanche, Matthew Booker, Bruno R. Steinkraus, Michael Schnall-Levin, Yong Zhao, Todd DeLuca, Fernando Bejarano, Zhe Han, Eric C. Lai, Dennis P. Wall, Norbert Perrimon () and David Van Vactor ()
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Tudor A. Fulga: Harvard Medical School
Elizabeth M. McNeill: Harvard Medical School
Richard Binari: Harvard Medical School
Julia Yelick: Harvard Medical School
Alexandra Blanche: Harvard Medical School
Matthew Booker: Harvard Medical School
Bruno R. Steinkraus: Weatherall Institute of Molecular Medicine, University of Oxford
Michael Schnall-Levin: Harvard Medical School
Yong Zhao: Harvard Medical School
Todd DeLuca: Harvard Medical School
Fernando Bejarano: Sloan-Kettering Institute
Zhe Han: Center for Cancer and Immunology Research, Children’s Research Institute, Children’s National Medical Center
Eric C. Lai: Sloan-Kettering Institute
Dennis P. Wall: Harvard Medical School
Norbert Perrimon: Harvard Medical School
David Van Vactor: Harvard Medical School

Nature Communications, 2015, vol. 6, issue 1, 1-10

Abstract: Abstract Although the impact of microRNAs (miRNAs) in development and disease is well established, understanding the function of individual miRNAs remains challenging. Development of competitive inhibitor molecules such as miRNA sponges has allowed the community to address individual miRNA function in vivo. However, the application of these loss-of-function strategies has been limited. Here we offer a comprehensive library of 141 conditional miRNA sponges targeting well-conserved miRNAs in Drosophila. Ubiquitous miRNA sponge delivery and consequent systemic miRNA inhibition uncovers a relatively small number of miRNA families underlying viability and gross morphogenesis, with false discovery rates in the 4–8% range. In contrast, tissue-specific silencing of muscle-enriched miRNAs reveals a surprisingly large number of novel miRNA contributions to the maintenance of adult indirect flight muscle structure and function. A strong correlation between miRNA abundance and physiological relevance is not observed, underscoring the importance of unbiased screens when assessing the contributions of miRNAs to complex biological processes.

Date: 2015
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8279

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DOI: 10.1038/ncomms8279

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