The molecular origin of high DNA-repair efficiency by photolyase

Chuang Tan, Zheyun Liu, Jiang Li, Xunmin Guo, Lijuan Wang, Aziz Sancar and Dongping Zhong ()
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Chuang Tan: and Programs of Biophysics, Chemical Physics, and Biochemistry, The Ohio State University
Zheyun Liu: and Programs of Biophysics, Chemical Physics, and Biochemistry, The Ohio State University
Jiang Li: and Programs of Biophysics, Chemical Physics, and Biochemistry, The Ohio State University
Xunmin Guo: and Programs of Biophysics, Chemical Physics, and Biochemistry, The Ohio State University
Lijuan Wang: and Programs of Biophysics, Chemical Physics, and Biochemistry, The Ohio State University
Aziz Sancar: University of North Carolina School of Medicine
Dongping Zhong: and Programs of Biophysics, Chemical Physics, and Biochemistry, The Ohio State University

Nature Communications, 2015, vol. 6, issue 1, 1-6

Abstract: Abstract The primary dynamics in photomachinery such as charge separation in photosynthesis and bond isomerization in sensory photoreceptor are typically ultrafast to accelerate functional dynamics and avoid energy dissipation. The same is also true for the DNA repair enzyme, photolyase. However, it is not known how the photoinduced step is optimized in photolyase to attain maximum efficiency. Here, we analyse the primary reaction steps of repair of ultraviolet-damaged DNA by photolyase using femtosecond spectroscopy. With systematic mutations of the amino acids involved in binding of the flavin cofactor and the cyclobutane pyrimidine dimer substrate, we report our direct deconvolution of the catalytic dynamics with three electron-transfer and two bond-breaking elementary steps and thus the fine tuning of the biological repair function for optimal efficiency. We found that the maximum repair efficiency is not enhanced by the ultrafast photoinduced process but achieved by the synergistic optimization of all steps in the complex repair reaction.

Date: 2015
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DOI: 10.1038/ncomms8302

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