CD24 tracks divergent pluripotent states in mouse and human cells

Nika Shakiba, Carl A. White, Yonatan Y. Lipsitz, Ayako Yachie-Kinoshita, Peter D Tonge, Samer M. I. Hussein, Mira C. Puri, Judith Elbaz, James Morrissey-Scoot, Mira Li, Javier Munoz, Marco Benevento, Ian M. Rogers, Jacob H. Hanna, Albert J. R. Heck, Bernd Wollscheid, Andras Nagy and Peter W Zandstra ()
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Nika Shakiba: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto
Carl A. White: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto
Yonatan Y. Lipsitz: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto
Ayako Yachie-Kinoshita: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto
Peter D Tonge: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital
Samer M. I. Hussein: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital
Mira C. Puri: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital
Judith Elbaz: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital
James Morrissey-Scoot: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto
Mira Li: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital
Javier Munoz: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht University for Pharmaceutical Sciences, Utrecht University
Marco Benevento: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht University for Pharmaceutical Sciences, Utrecht University
Ian M. Rogers: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital
Jacob H. Hanna: Weizmann Institute of Science
Albert J. R. Heck: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht University for Pharmaceutical Sciences, Utrecht University
Bernd Wollscheid: Institute of Molecular Systems Biology, Swiss Federal Institute of Technology (ETH) Zürich
Andras Nagy: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital
Peter W Zandstra: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto

Nature Communications, 2015, vol. 6, issue 1, 1-11

Abstract: Abstract Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.

Date: 2015
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DOI: 10.1038/ncomms8329

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