Steady-state cross-correlations for live two-colour super-resolution localization data sets

Matthew B. Stone and Sarah L. Veatch ()
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Matthew B. Stone: University of Michigan
Sarah L. Veatch: University of Michigan

Nature Communications, 2015, vol. 6, issue 1, 1-10

Abstract: Abstract Cross-correlation of super-resolution images gathered from point localizations allows for robust quantification of protein co-distributions in chemically fixed cells. Here this is extended to dynamic systems through an analysis that quantifies the steady-state cross-correlation between spectrally distinguishable probes. This methodology is used to quantify the co-distribution of several mobile membrane proteins in both vesicles and live cells, including Lyn kinase and the B-cell receptor during antigen stimulation.

Date: 2015
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DOI: 10.1038/ncomms8347

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