Ligand regulation of a constitutively dimeric EGF receptor

Daniel M. Freed, Diego Alvarado and Mark A. Lemmon ()
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Daniel M. Freed: University of Pennsylvania Perelman School of Medicine, 322A Clinical Research Building, 415 Curie Boulevard, Philadelphia, Pennsylvania 19104-6059, USA
Diego Alvarado: University of Pennsylvania Perelman School of Medicine, 322A Clinical Research Building, 415 Curie Boulevard, Philadelphia, Pennsylvania 19104-6059, USA
Mark A. Lemmon: University of Pennsylvania Perelman School of Medicine, 322A Clinical Research Building, 415 Curie Boulevard, Philadelphia, Pennsylvania 19104-6059, USA

Nature Communications, 2015, vol. 6, issue 1, 1-7

Abstract: Abstract Ligand-induced receptor dimerization has traditionally been viewed as the key event in transmembrane signalling by epidermal growth factor receptors (EGFRs). Here we show that the Caenorhabditis elegans EGFR orthologue LET-23 is constitutively dimeric, yet responds to its ligand LIN-3 without changing oligomerization state. SAXS and mutational analyses further reveal that the preformed dimer of the LET-23 extracellular region is mediated by its domain II dimerization arm and resembles other EGFR extracellular dimers seen in structural studies. Binding of LIN-3 induces only minor structural rearrangements in the LET-23 dimer to promote signalling. Our results therefore argue that EGFR can be regulated by allosteric changes within an existing receptor dimer—resembling signalling by insulin receptor family members, which share similar extracellular domain compositions but form covalent dimers.

Date: 2015
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DOI: 10.1038/ncomms8380

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