Opposing calcium-dependent signalling pathways control skeletal muscle differentiation by regulating a chromatin remodelling enzyme

Brian T. Nasipak, Teresita Padilla-Benavides, Karin M. Green, John D. Leszyk, Wenjie Mao, Silvana Konda, Saïd Sif, Scott A. Shaffer, Yasuyuki Ohkawa and Anthony N. Imbalzano ()
Additional contact information
Brian T. Nasipak: University of Massachusetts Medical School
Teresita Padilla-Benavides: University of Massachusetts Medical School
Karin M. Green: Proteomics and Mass Spectrometry Facility, University of Massachusetts Medical School
John D. Leszyk: Proteomics and Mass Spectrometry Facility, University of Massachusetts Medical School
Wenjie Mao: University of Massachusetts Medical School
Silvana Konda: University of Massachusetts Medical School
Saïd Sif: College of Medicine, Ohio State University
Scott A. Shaffer: Proteomics and Mass Spectrometry Facility, University of Massachusetts Medical School
Yasuyuki Ohkawa: University of Massachusetts Medical School
Anthony N. Imbalzano: University of Massachusetts Medical School

Nature Communications, 2015, vol. 6, issue 1, 1-12

Abstract: Abstract Calcium signalling is important for differentiation-dependent gene expression, but is also involved in other cellular functions. Therefore, mechanisms must exist to distinguish calcium signalling relevant to differentiation. Calcineurin is a calcium-regulated phosphatase that is required for myogenic gene expression and skeletal muscle differentiation. Here, we demonstrate that inhibition of calcineurin blocks chromatin remodelling and that the Brg1 ATPase of the SWI/SNF chromatin remodelling enzyme, which is required for the activation of myogenic gene expression, is a calcineurin substrate. Furthermore, we identify the calcium-regulated classical protein kinase C β (PKCβ) as a repressor of myogenesis and as the enzyme that opposes calcineurin function. Replacement of endogenous Brg1 with a phosphomimetic mutant in primary myoblasts inhibits myogenesis, whereas replacement with a non-phosphorylatable mutant allows myogenesis despite inhibition of calcineurin signalling, demonstrating the functionality of calcineurin/PKC-modified residues. Thus, the Brg1 chromatin remodelling enzyme integrates two antagonistic calcium-dependent signalling pathways that control myogenic differentiation.

Date: 2015
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms8441 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8441

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms8441

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().