Structure and gating of the nuclear pore complex

Matthias Eibauer, Mauro Pellanda, Yagmur Turgay, Anna Dubrovsky, Annik Wild and Ohad Medalia ()
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Matthias Eibauer: University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland
Mauro Pellanda: University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland
Yagmur Turgay: University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland
Anna Dubrovsky: University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland
Annik Wild: University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland
Ohad Medalia: University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland

Nature Communications, 2015, vol. 6, issue 1, 1-9

Abstract: Abstract Nuclear pore complexes (NPCs) perforate the nuclear envelope and allow the exchange of macromolecules between the nucleus and the cytoplasm. To acquire a deeper understanding of this transport mechanism, we analyse the structure of the NPC scaffold and permeability barrier, by reconstructing the Xenopus laevis oocyte NPC from native nuclear envelopes up to 20 Å resolution by cryo-electron tomography in conjunction with subtomogram averaging. In addition to resolving individual protein domains of the NPC constituents, we propose a model for the architecture of the molecular gate at its central channel. Furthermore, we compare and contrast this native NPC structure to one that exhibits reduced transport activity and unveil the spatial properties of the NPC gate.

Date: 2015
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DOI: 10.1038/ncomms8532

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