Human Upf1 is a highly processive RNA helicase and translocase with RNP remodelling activities

Francesca Fiorini, Debjani Bagchi, Hervé Le Hir () and Vincent Croquette ()
Additional contact information
Francesca Fiorini: Institut de Biologie de l’Ecole Normale Supérieure, CNRS UMR8197
Debjani Bagchi: Institut de Biologie de l’Ecole Normale Supérieure, CNRS UMR8197
Hervé Le Hir: Institut de Biologie de l’Ecole Normale Supérieure, CNRS UMR8197
Vincent Croquette: Institut de Biologie de l’Ecole Normale Supérieure, CNRS UMR8197

Nature Communications, 2015, vol. 6, issue 1, 1-10

Abstract: Abstract RNA helicases are implicated in most cellular RNA-dependent events. In eukaryotes however, only few have been functionally characterized. Upf1 is a RNA helicase essential for nonsense-mediated mRNA decay (NMD). Here, using magnetic tweezers and bulk assays, we observe that human Upf1 is able to translocate slowly over long single-stranded nucleic acids with a processivity >10 kb. Upf1 efficiently translocates through double-stranded structures and protein-bound sequences, demonstrating that Upf1 is an efficient ribonucleoprotein complex remodeler. Our observation of processive unwinding by an eukaryotic RNA helicase reveals that Upf1, once recruited onto NMD mRNA targets, can scan the entire transcript to irreversibly remodel the mRNP, facilitating its degradation by the NMD machinery.

Date: 2015
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms8581 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8581

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms8581

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().