Genome-wide microRNA screening reveals that the evolutionary conserved miR-9a regulates body growth by targeting sNPFR1/NPYR

Yoon Seok Suh, Shreelatha Bhat, Seung-Hyun Hong, Minjung Shin, Suhyoung Bahk, Kyung Sang Cho, Seung-Whan Kim, Kyu-Sun Lee, Young-Joon Kim, Walton D. Jones () and Kweon Yu ()
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Yoon Seok Suh: Neurophysiology Research Group, Bio-Nano Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yusong-gu, Daejeon 305-806, Korea
Shreelatha Bhat: Korea Advanced Institute of Science and Technology (KAIST)
Seung-Hyun Hong: Neurophysiology Research Group, Bio-Nano Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yusong-gu, Daejeon 305-806, Korea
Minjung Shin: Korea Advanced Institute of Science and Technology (KAIST)
Suhyoung Bahk: Korea Advanced Institute of Science and Technology (KAIST)
Kyung Sang Cho: Konkuk University
Seung-Whan Kim: Chungnam National University Hospital
Kyu-Sun Lee: Neurophysiology Research Group, Bio-Nano Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yusong-gu, Daejeon 305-806, Korea
Young-Joon Kim: School of Life Sciences, Gwangju Institute of Science and Technology (GIST)
Walton D. Jones: Korea Advanced Institute of Science and Technology (KAIST)
Kweon Yu: Neurophysiology Research Group, Bio-Nano Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yusong-gu, Daejeon 305-806, Korea

Nature Communications, 2015, vol. 6, issue 1, 1-11

Abstract: Abstract MicroRNAs (miRNAs) regulate many physiological processes including body growth. Insulin/IGF signalling is the primary regulator of animal body growth, but the extent to which miRNAs act in insulin-producing cells (IPCs) is unclear. Here we generate a UAS-miRNA library of Drosophila stocks and perform a genetic screen to identify miRNAs whose overexpression in the IPCs inhibits body growth in Drosophila. Through this screen, we identify miR-9a as an evolutionarily conserved regulator of insulin signalling and body growth. IPC-specific miR-9a overexpression reduces insulin signalling and body size. Of the predicted targets of miR-9a, we find that loss of miR-9a enhances the level of sNPFR1. We show via an in vitro binding assay that miR-9a binds to sNPFR1 mRNA in insect cells and to the mammalian orthologue NPY2R in rat insulinoma cells. These findings indicate that the conserved miR-9a regulates body growth by controlling sNPFR1/NPYR-mediated modulation of insulin signalling.

Date: 2015
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DOI: 10.1038/ncomms8693

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