An actin-dependent spindle position checkpoint ensures the asymmetric division in mouse oocytes

Aïcha Metchat (), Manuel Eguren, Julius M. Hossain, Antonio Z. Politi, Sébastien Huet and Jan Ellenberg
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Aïcha Metchat: European Molecular Biology Laboratory
Manuel Eguren: European Molecular Biology Laboratory
Julius M. Hossain: European Molecular Biology Laboratory
Antonio Z. Politi: European Molecular Biology Laboratory
Sébastien Huet: European Molecular Biology Laboratory
Jan Ellenberg: European Molecular Biology Laboratory

Nature Communications, 2015, vol. 6, issue 1, 1-10

Abstract: Abstract Faithful chromosome segregation, during meiosis, is of critical importance to prevent aneuploidy in the resulting embryo. In mammalian oocytes, the segregation of homologous chromosomes takes place with the spindle located at the cell’s periphery. The spindle is often assembled close to the centre of the cell, which necessitates the actin network for spindle transport to the cell cortex. In this study, we investigate how the segregation of chromosomes is coordinated with the positioning of the metaphase I spindle. We develop different assays to perturb the spindle’s position and to delay its relocation to the cell periphery. We find that anaphase is delayed until the spindle is positioned in close proximity with the oocyte cortex. We further show that the metaphase arrest is dependent on a functional actin network, in addition to the spindle assembly checkpoint. Our work provides the first evidence for the existence of a functional spindle position checkpoint.

Date: 2015
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DOI: 10.1038/ncomms8784

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