Condensin targets and reduces unwound DNA structures associated with transcription in mitotic chromosome condensation

Takashi Sutani (), Toyonori Sakata, Ryuichiro Nakato, Koji Masuda, Mai Ishibashi, Daisuke Yamashita, Yutaka Suzuki, Tatsuya Hirano, Masashige Bando and Katsuhiko Shirahige ()
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Takashi Sutani: Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo
Toyonori Sakata: Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo
Ryuichiro Nakato: Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo
Koji Masuda: Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo
Mai Ishibashi: Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo
Daisuke Yamashita: Chromosome Dynamics Laboratory, RIKEN
Yutaka Suzuki: Graduate School of Frontier Sciences, The University of Tokyo
Tatsuya Hirano: Chromosome Dynamics Laboratory, RIKEN
Masashige Bando: Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo
Katsuhiko Shirahige: Research Center for Epigenetic Disease, Institute of Molecular and Cellular Biosciences, The University of Tokyo

Nature Communications, 2015, vol. 6, issue 1, 1-13

Abstract: Abstract Chromosome condensation is a hallmark of mitosis in eukaryotes and is a prerequisite for faithful segregation of genetic material to daughter cells. Here we show that condensin, which is essential for assembling condensed chromosomes, helps to preclude the detrimental effects of gene transcription on mitotic condensation. ChIP-seq profiling reveals that the fission yeast condensin preferentially binds to active protein-coding genes in a transcription-dependent manner during mitosis. Pharmacological and genetic attenuation of transcription largely rescue bulk chromosome segregation defects observed in condensin mutants. We also demonstrate that condensin is associated with and reduces unwound DNA segments generated by transcription, providing a direct link between an in vitro activity of condensin and its in vivo function. The human condensin isoform condensin I also binds to unwound DNA regions at the transcription start sites of active genes, implying that our findings uncover a fundamental feature of condensin complexes.

Date: 2015
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8815

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DOI: 10.1038/ncomms8815

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